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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important position in marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two further splice variants had been described but in-depth nucleotide sequence details just isn't nevertheless readily available.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified because of the antibody.
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This guide outlines more doable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By comparing the staining from your blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Data Cyclin E belongs for the extremely conserved cyclin family, whose customers are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show unique expression and degradation styles which contribute into the temporal coordination of every mitotic party. Cyclin E kinds a posh with and functions for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S section boundary and it is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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